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Cloning and Expression
Transfection Kits and Reagents
Transfection Reagents
ViaFect Transfection Reagent
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ViaFect Transfection Reagent
ViaFect Transfection Reagent
ViaFect Transfection Reagent from Promega
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Citations:
(134)
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ViaFect Transfection Reagent
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Description
ViaFect Transfection Reagent is a novel formulation reagent designed to efficiently introduce DNA into a wide variety of cell lines
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
ViaFect Transfection Reagent
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
E4982
Quantity
2 x 0.75ml
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (134)
(41)
FOXA1 regulates alternative splicing in prostate cancer
Cell reports
September 27, 2022
Del Giudice M, Foster JG, Peirone S, Rissone A, Caizzi L, Gaudino F, Parlato C, Anselmi F, Arkell R, Guarrera S,
et al
.
plasmid DNA and siRNA duplexes (Table S1) were performed as detailed in the figure legends using ViaFect (
E4981
,
Promega
) and RNAiMax (
13778-075
,
Thermo Fisher Scientific
), respectively, according to the manufacturers’
(42)
Conserved features of TERT promoter duplications reveal an activation mechanism that mimics hotspot mutations in cancer
Nature Communications
September 16, 2022
Carter J. Barger, Abigail K. Suwala, Katarzyna M. Soczek, Albert S. Wang, Min Y. Kim, Chibo Hong, Jennifer A. Doudna, Susan M. Chang, Joanna J. Phillips, David A. Solomon,
et al.
reporter assay
Transfection
of TERTp reporter plasmids was carried out with
ViaFect transfection reagent
(
Promega
, #
E4981
). Briefly, cells were seeded at a density of 3000 or 6000 cells per well in a 96-well clear bottom white
(43)
A RUNX-targeted gene switch-off approach modulates the BIRC5/PIF1-p21 pathway and reduces glioblastoma growth in mice
Communications biology
September 9, 2022
Hattori EY, Masuda T, Mineharu Y, Mikami M, Terada Y, Matsui Y, Kubota H, Matsuo H, Hirata M, Kataoka TR,
et al
.
expression vector (RIKEN BRC) tet-inducibly expressing
RUNX1
cDNA using
ViaFect Transfection Reagent
(Cat#
E4981
;
Promega
) 48 h after
RUNX1
expression. Promoter activities were measured 16 h after
transfection
using
(44)
Sublethal cytochrome c release generates drug-tolerant persister cells
Cell
September 1, 2022
Kalkavan H, Chen MJ, Crawford JC, Quarato G, Fitzgerald P, Tait SWG, Goding CR, Green DR.
"
Fugene HD Transfection Reagent
,
Promega
, FuGENE HD
Transfection
",
Promega
,Cat#
E2311
ViaFect Transfection Reagent
,
Promega
,Cat#
E4981
Lipofectamine™ RNAiMAX Transfection Reagent
,Therno Scientific,Cat#
13778075
Ercc RNA Spike-In Mix
,
Thermo Scientific
(45)
Myxoma virus lacking the host range determinant M062 stimulates cGAS-dependent type 1 interferon response and unique transcriptomic changes in human monocytes/macrophages
PLoS pathogens
September 1, 2022
Conrad SJ, Raza T, Peterson EA, Liem J, Connor R, Nounamo B, Cannon M, Liu J.
instructions.
Transfection
Transfection
of plasmid DNA is performed using
ViaFect transfection reagent
(
Promega
, Catalog #
E4981
) based on recommendation of manufacturer’s standard protocol and transfectagro Reduced Serum Medium
(46)
Pharmacological inhibition of ALCAT1 mitigates amyotrophic lateral sclerosis by attenuating SOD1 protein aggregation
Molecular Metabolism
September 1, 2022
Xueling Liu, Jun Zhang, Jie Li, Chengjie Song, Yuguang Shi
CRISPR/Cas9
plasmids (
sc-432
,509 and
sc-432509-HDR
) were from
Santa Cruz
. Viafect
transfection
regent (
E4982
) was obtained from
Promega
. Hoechst 33,342 solution (62,249), H2DCFDA (D399),
SuperScript™ IV first strand synthesis system
(47)
Alzheimer’s Amyloid-β Accelerates Cell Senescence and Suppresses the SIRT1/NRF2 Pathway in Human Microglial Cells
Oxidative Medicine and Cellular Longevity
August 17, 2022
Yuqian An, Yi Li, Yujun Hou, Shichao Huang, Gang Pei
well into 6 cm dish.
SIRT1
plasmid or FUGW plasmid was
transfected
using ViaFect reagent (
Promega
, #
E4981
) according to manufacturer's instructions. After
transfection
for 24 h, cells were stimulated by Aβ
(48)
Impact of DYRK1A Expression on TNNT2 Splicing and Daunorubicin Toxicity in Human iPSC-Derived Cardiomyocytes
Cardiovascular Toxicology
August 1, 2022
Romina Beatriz Cejas, Miriam Tamaño-Blanco, John Edgar Fontecha, Javier Guillermo Blanco
6 post initial plating were
transfected
with 100 ng plasmid DNA using
ViaFect transfection reagent
(
E4981
,
Promega
) and the following
human
DYRK1A
and
SRSF6
expression constructs:
DYRK1A
human
clone
(NM_001396,
(49)
Structure and regulation of the nuclear exosome targeting complex guides RNA substrates to the exosome
Molecular Cell
July 7, 2022
Piotr Gerlach, William Garland, Mahesh Lingaraju, Anna Salerno-Kochan, Fabien Bonneau, Jérôme Basquin, Torben Heick Jensen, Elena Conti
,
Thermo Fisher
,
10009D
Lipofectamine 3000 Transfection Reagent
,
Thermo Fisher
,L300001
Viafect Transfection Reagent
,
Promega
,
E4981
NEBuilder HiFi DNA Assembly cloning kit
,
NEB
,
E5520S
GeneJET PCR Purification Kit
,
Thermo Fisher
,
K0701
,, Deposited
(50)
De novo labeling and trafficking of individual lipid species in live cells
Molecular Metabolism
July 1, 2022
Jun Zhang, Jia Nie, Haoran Sun, Jie Li, John-Paul Andersen, Yuguang Shi
cells by transfecting
CRISPR-Cas9
mouse
plasmids from Santa Cruz using
Viafect Transfection Reagent
(#
E4982
,
Promega
), and then purified through GFP and RFP
fluorescence
by the UT Health San Antonio
Flow Cytometry
(51)
Puerarin attenuates diabetic kidney injury through interaction with Guanidine nucleotide‐binding protein Gi subunit alpha‐1 (Gnai1) subunit
Journal of Cellular and Molecular Medicine
July 1, 2022
Qingqing Zhu, Shumin Yang, Chengguo Wei, Geming Lu, Kyung Lee, John Cijiang He, Ruijie Liu, Yifei Zhong
with 5 μg of empty vector or corresponding vectors as indicated in the figures using Viafect reagent (
E4981
;
Promega
, Madison, WI) according to the manufacturer's protocol. 2.9
Western blot
Cells were lysed
(52)
Hypoxia-directed tumor targeting of CRISPR-Cas9 and HSV-TK suicide gene therapy using lipid nanoparticles
Molecular Therapy - Methods & Clinical Development
June 9, 2022
Alicia Davis, Kevin V. Morris, Galina Shevchenko
cells
Transfection
Plasmid transfection
was carried out using
Lipofectamine 3000
(
Invitrogen
11668019
) or ViaFect (
Promega
E4981
) according to the protocol provided by the manufacturer. A complete list of plasmids used in this study
(53)
Intron retention by a novel intronic mutation in
DKC1
gene caused recurrent still birth and early death in a Chinese family
Molecular Genetics & Genomic Medicine
June 1, 2022
Qiufang Guo et al.
containing 5% CO2.
Transfection
was performed according to the manufacturer's instructions (
Promega
,
E4981
), then
transfected
cells were incubated for 24 or 48 hr. To block nonsense‐mediated mRNA decay (NMD),
(54)
Small molecules targeting the NADH-binding pocket of VDAC modulate mitochondrial metabolism in hepatocarcinoma cells
Biomedicine & Pharmacotherapy
June 1, 2022
Kareem A. Heslop et al.
the following combinations: VDAC1/VDAC2, VDAC1/VDAC3, and VDAC2/ VDAC3 using Viafect (
Promega
, Cat. #
E4981
), according to the manufacturer’s protocol. After 24 h, the media was changed to EMEM, 10% FBS + puromycin
(55)
TP53-inducible putative long noncoding RNAs encode functional polypeptides that suppress cell proliferation
Genome research
June 1, 2022
Xu W, Liu C, Deng B, Lin P, Sun Z, Liu A, Xuan J, Li Y, Zhou K, Zhang X,
et al
.
h for
RNA-seq
and
Ribo-seq
. The cells were
transfected
with plasmids using ViaFect reagent (
Promega
E4982
) according to the manufacturer's instructions. Construction of stable
TP53
, TP53LC02, and TP53LC04-silenced
(56)
Complementary omics strategies to dissect p53 signaling networks under nutrient stress
Cellular and molecular life sciences : CMLS
May 30, 2022
Galhuber M, Michenthaler H, Heininger C, Reinisch I, Nössing C, Krstic J, Kupper N, Moyschewitz E, Auer M, Heitzer E,
et al
.
collagen-coated T75 flasks (approx. 1.5 × 107 cells) and transiently
transfected
(
VIAfect transfection reagent
,
E4981
,
Promega
) with either V5-
p53
-miniTurbo-NES or V5-EGFP-miniTurbo-NES. After 24 h of
transfection
, intracellular
(57)
Chromatin modifier HUSH co-operates with RNA decay factor NEXT to restrict transposable element expression
Molecular cell
May 5, 2022
Garland W, Müller I, Wu M, Schmid M, Imamura K, Rib L, Sandelin A, Helin K, Jensen TH.
10009D
Lipofectamine 3000 Transfection Reagent
,
Thermo Fisher
,Cat# L300001
Viafect Transfection Reagent
,
Promega
,Cat#
E4981
Benzonase nuclease,Millipore,Cat# 70746 Micrococcal Nuclease (MNase),Sigma-Aldrich,Cat# N3755 RNaseA,Sigma-Aldrich,Cat#
(58)
Combination of CENP-B Box Positive and Negative Synthetic Alpha Satellite Repeats Improves De Novo Human Artificial Chromosome Formation
Cells
April 19, 2022
Koei Okazaki, Megumi Nakano, Jun-ichirou Ohzeki, Koichiro Otake, Kazuto Kugou, Vladimir Larionov, William C. Earnshaw, Hiroshi Masumoto
previously [45,46]. FuGENE HD (Promega Corp., Madison, WI, USA;
E2312
) or Viafect (
Promega
, Beijing, China;
E4981
) was used as the
transfection reagent
. For transient protein tethering to the synthetic alphoid, 750
(59)
Differential expression of members of SOX family of transcription factors in failing human hearts
Translational Research
April 1, 2022
Chia-Feng Liu, Ying Ni, Varun Thachil, Michael Morley, Christine S. Moravec, Wai Hong Wilson Tang
and a combination of plasmids expressing SOX4 or
SOX8
using
ViaFect Transfection reagent
(
Promega
,
E4981
). Cells were harvested 24 hr after
transfection
, and luciferase activity was determined using
Firefly & Renilla Luciferase Assay Kit
(60)
A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development
Communications biology
March 8, 2022
Reyes-Alcaraz A, Lucero Garcia-Rojas EY, Merlinsky EA, Seong JY, Bond RA, McConnell BK.
µg of β2AR-SmBiT plus 1 µg of LgBiT-FYVE and
ViaFect™ Transfection Reagent
(
Promega
, catalog number
E4981
). Twenty-four hours later, on the day of imaging, medium was removed, and cells were incubated with
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Reviews
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(1)
6/17/2019
Easy-to-Use Transfection Reagent with Good Results from Promega
Wei Yang Sit
Highly recommended transfection reagent for those who intend to carry out transfection.
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ViaFect Transfection Reagent from Promega
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